Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPIVIIGSGMAGYTLAREFRKLNPEQELVMICADDAVNYAKPTLSNAFAGKKAPEQIPLGDAAKMSAQLNMRIEPFTWVKEILAERHELVLEKDGIISQQPYSKLILAVGANPIRLAIAGDGSDDIHVVNSLIDYRSFRENLAQRKDKRVVILGAGLIGCEFANDLLHSEYDVTVIDLAPQPLGRLLPSHIASAFQQNLEEAGVKFTLGTTVEKVSKINNGEDYAVTLANGQTLVADIVLSAIGLQPNISLAQSANIQTSRGVITNSLLETNQADIYAIGDCAEVNGT-----LLPYVMPIMQQARALAKTLSGQQTNVHYPAMPV-AVKTPAAPLTVLPAPVDVDVNWETEEFD-DGMLAKAIDNEGTLRGFVLLGATAGKQRLTLTKLVPDLIPAQA 4EMJ Chain:A ((2-370)) ATHVAIIGNGVGGFTTAQALRAEGFEGRISLIGDEPHLPYDRPSLSKAVLDGSLERPPILA-EADWYGEARIDMLTGPEVTALDVQTRTISLDDG---TTLSADAIVIATGSRARTMALPGSQLPGVVTLRTYGDVQVLRDSWTS--ATRLLIVGGGLIGCEVATTARKLGLSVTILEAGDELLVRVLGRRIGAWLRGLLTELGVQVELGTGVVGFSGE--GQLEQVMASDGRSFVADSALICVGAEPADQLARQAGLACDRGVIVDHCGATLAKGVFAVGDVASWPLRAGGRRSLETYMNAQRQAAAVAAAILGKNVSA--PQLPVSWTEIAGHRMQMAGDIEGPGD-FVSRGMPGSGAALLFRLQERRIQAVVAVDAP--------------------

General information: TITO was launched using: RESULT: Template: 4EMJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2159 -237517 -110.01 -656.12 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -110.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4EMJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EMJ-query.scw PDB file : Tito_Scwrl_4EMJ.pdb: