Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAARACGLDNMQNAQRLAAIIEMLHTATLVHDDV--VDESGLRRGRPTANATWNNQTAVLVGDFLIARAFDLLVDLNN-------MTLLKDF--STGTCEIAEGEVLQLQSQHQPDTTEETYLKIIHGKTSRLFELATEGAAILAG-QEAYREPLRLFAGHFGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPTLPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQALSILPDTPYRQALINLTRLALHRIQ 3KRF Chain:D ((41-249)) --------------------------------------------AGGKRVRPMLCIAACELVGGDE-STAMPAACAVEMIHTMSLMHDDLPCMDNDDLRRGKPTNHMAFGESVAVLAGDALLSFAFEHVAAATKGAPPERIVRVLGELAVSIGSEGLVAGQVVDVCSEGMAEVGLDHLEFIHHHKTAALLQGSVVLGAILGGGKEEEVAKLRKFANCIGLLFQVVDDILDVTKSSKELGKTAGKDLVADKTTYP-----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3KRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 872 -9026 -10.35 -45.82 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -10.35 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_3KRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KRF-query.scw PDB file : Tito_Scwrl_3KRF.pdb: