Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKLIVLSNRISMPSGK-ASAGGLAVAVQDALNDSNGIWLGWNGQQITDTEAPEFDQAYSHGIDYITCPLTHQQYAQYYCGFANKVLWPAMHDRDDLIEYNAEEYNTYQKVNRLFAEKLQQIAQPDDLIWVHDYHFFSVARHCRELGMQNKIGFFLHIPFASLNIWRKIPVALQLVQDLCQYDVIGLQTQTDQNTCMQTCMGLLEAQKI----LSDRISYKKRQILIKSYPIGVQPELIQRQAQQAFHTPYVFNFED-IPRQKTIIGVDRIDYSKGLLERFNAFATFLETNPEYHGLVRHLQVATPSRTDIPAYQRLYQRFKAKLELINEEFAHEDWRPIDCCFDTVQHDSLMHIYRRSDICWISSLRDGMNLVAKEYIAAQDPENPGVLILSKYAGAAEQMTQALIVDPLDRAAMMDSLKTALEMSKAERINRYEQLIEGLAATDLSDWRNNFLKDLENSPTFTKSKLSLQEKYQPTYHAL 5V0T Chain:E ((10-466)) -GRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKV-EERGPVTFATIALMRRDYDQYYRGFSNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVPLLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPASQVLLAVPPHRELVEALCSFDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAELAKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFLQIAPPTRADMHAYQDIRLQLEGESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQELDGALIVNPVDIDGMAEALARALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQ-----------------------

General information: TITO was launched using: RESULT: Template: 5V0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2650 -3518 -1.33 -7.80 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.80 3D Compatibility (PKB) : -1.33 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_5V0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5V0T-query.scw PDB file : Tito_Scwrl_5V0T.pdb: