Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEKGVETMKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKKYLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAG-NTGALLAAG------LFIVGRIKNIERPGLMSTLPVVGKEGAGFDML------DLGANAENKPEHLLQYGILGSFYARKVRGIGRPRVALLN----NGTEATKGSE---VTKKAYELLQNETSL-NFIGNVEARELLN----GVADVVVT-DGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 2HI1 Chain:B ((103-279)) -------------------------------------------------------------------------------------CVKRATELALRGDVQAIATAPLNKEALHLAGHNYPGHTELLATLTHSRD-YAM-VLYTDKL--KVIHVSTHIALRKFL--DTLSTARVETVIGIADTFLKR-VGYVKPRIAVAGVNPHAG---LFGDEETRILTPAITDARAK--GMDVYGPCPPDTVFLQAYEGQYDMVVAMYHDQGHIPLKLL-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2HI1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 475 4907 10.33 33.38 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 10.33 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_2HI1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HI1-query.scw PDB file : Tito_Scwrl_2HI1.pdb: