Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFKVKNV---D---NYAGHIDLGEGDETLGIFGHMDVVPAGD---GWD----TDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESG-----WGDMAYYFEH---------EEEPDFGFSPDAEFP-IINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKG-AHGA------SPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEE----NGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTR-GARVMEPHYVPMDDPLVATLLQVYEDHTGEKGY-EQ--IIGGGTYGRLLK----RGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 5K8O Chain:D ((7-422)) -VAKVMKTLDGMREGLIQTAVELGSIEAPTGR------------EGAAGDYVYEWMARNGFGPERVGVFDDRFNVVGRLRGTGGGASLSFNSHLDTIMAREDTARFADANDRIYHEAWHEEGRIYGYSVVNCKGPMACWLIAAKALKEAGAALKGDVVLTAVCGEIDCEPVDEFQGHDYLAEDIGARYAISHGAISDYALVAEATNFKPAWV---------------------------------------------------------------------EAGKVFLKVTVFAGPSRYTPYVPRPVAALDSPNAIVRMAKLVEALEEW------ADNYEK----RYT------REYGGGTVVPKVAIGAIRGGVPYKIYRFPELCSIYMDIRLNPDTNPLVVQREVEAVVSKLGLKAEVKPFLFRRGYEAQGIEPLQNALEVAHREVVGRPTERPGSPECSMWRDTNPYNELGIPSLTYGCGG-----GAGGGNTYFLVDDMLKAAKVYAMTAMDLCN

General information: TITO was launched using: RESULT: Template: 5K8O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1877 19716 10.50 53.43 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : 10.50 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_5K8O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8O-query.scw PDB file : Tito_Scwrl_5K8O.pdb: