Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MYKMTRRSTMLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTNDLECITDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFFNVLGHYLSGEHNWSNIADTTFISLSKVYNLRNELINFFKQMQYLNADGHFEIPEKDYRSLLLTIIYETNRTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFSQEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKDLNVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKTYLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRK--LTISLSLLNEHKRKSPIEVFIVAPSDFRYLYYRQQLEDILGEHFSISNIICKQLREIVDDTFFCTQRIILCDSSLYQEGLGSEKTIIYPITFQTIHTVIDQLNKQI 4QVG Chain:A ((4-344)) ELSALVPVLFGHAAFQQLNAGCQLGLFELLHERGPLSAEEVADALRL------------------------PRRSADILLLGTTALGLSTVTD-----------GGYRNGAPIGAAFRDGLWPVLRDIVQYQDKIAYQ---------------PAADYVESLRT-----------------------------------------------GQNAGIRHFPGTTRDLYSRLAAVPGLEELFYRGMHAWSQLSNPVLLAQPDFTRVHRVLDVGGGDAVNAVALARAHPSL-RVTVLDRPGALEVARKTIAEAGLEER------------VRTHAADIF----TDSY---PAGHDCVLFAHQLVIWSPEQNLTLLRKAYDAVEPGGRVLVFNAFTDDDRTGP-----------LYAALDNVYFTTLPFRHST------------------IHRWADCESWLREAGF--TDVGRTAPPGWTPHGVVSGSRPR-

General information: TITO was launched using: RESULT: Template: 4QVG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1603 -10143 -6.33 -30.01 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -6.33 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_4QVG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QVG-query.scw PDB file : Tito_Scwrl_4QVG.pdb: