Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------MNDFTTEIVQTLVT---KGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVT--------MSEIADLIEKMYGHHYTPQTMSNMTKV---------LTEEVNAFKSR----ALNDKYVAIFMDATYIP-LKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELL------QDINSRGVQEVLLFITDGL----------------KGMKDTIHQIYPKAKY---QHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTF---YDFPPSIRRTIYS-TNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKRFSTGNRYISFNVY 5L3T Chain:B ((1-455)) MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTRFKIMCTSFLNYAR-DVDPWSAWSS------SDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAFVQFNE--AFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWSVLYKHVRYGNIQGVSLWLRQNERHLCA---RQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQN--KLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQLQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW

General information: TITO was launched using: RESULT: Template: 5L3T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1548 30300 19.57 80.37 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 19.57 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_5L3T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3T-query.scw PDB file : Tito_Scwrl_5L3T.pdb: