Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGDYIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKLVLPLDHSKIKGLNNIDPRFLD-L--DFDPDNQYSIPYFWGTLGIVYNDKFFDAN----QIKHWDDLWKPELKDSL-----MLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVADEIKMYMINEEASVAVTFSGEAADMMW------ENEHLHYVIPPEGSNLWFDNIVMPKTVKNKEGAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVYKDLGSKYLGIYNDLFLEFKMYRR 3TTN Chain:B ((7-339)) ------------------------------------LHIYNWTDYIAPTTLKDFTKESGIDVSYDVFDSNETLEGKLVS---GYDIVVPSNNFLGKQIQAGAFQKLDKSKLPNWKNLDPALLKQLEVSD-PGNQYAVPYLWGTNGIGYNVAKVKEVLGDQPIDSWAILFEPENMKKLAKCGVAFMDSGDEMLPAALNYLGLDPNTHDPKDYKKAEEVLTKVRPYVSYFHSSKYISDLANGNICVAFGYSGDVFQAAARAEEAGKGIDIQYVIPKEGANLWFDLMAIPADAKAADNAYAFIDYLLRPEVIAKVSDYVGYANAIPGARPLMDKSVSDSEEVYPPQAVLDKLYVSAVLPAKVLRLQTRTWTRI-----

General information: TITO was launched using: RESULT: Template: 3TTN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1733 -5636 -3.25 -18.06 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -3.25 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3TTN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TTN-query.scw PDB file : Tito_Scwrl_3TTN.pdb: