Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLS-GELDYRYEKDVHPSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILK--HPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLSGITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDFNAGVLL----EQPMEVILESFVQK---IIAVASGEETKNEQNDVREIAIFKNGVTL--------------------- 4OFZ Chain:A ((85-513)) QTVDDFKNLMYKMQETRRAIVFALL----NEKDLT----------------KDDVEILKRAYEKLTDNFQ-----------REMCTLTTKLSVNIGDETRGLEKDLKYLDALMNIRREEPNL-----------------------LWPIIMSRV----------DLFSILANYHPKGKETFLKEYEDTVKFLKTFISSEAITGKKPIFITDWDGTM---------------KDYCSQ--YATNLQPVYSAVGMTRFAASFTRISAVLTAGPLR-----GPGILDLTAMPIDGPV--MFSGSWGREWWLS------GKRVVHQDGITDEGFNALQRLDDEMKDLL-------HTSPFALVGSGVQRKVDRLTLGVQTV--------CHHVTSELSNRYQMAVKERMHNSQILVFDPSTELEVEVVAHNSGIIWN------KGNGVERLIKSLGDSLQSPGKILICGDTLSDIPMVRQAVKQNPDGVLAIFVGAK-MSLREEVKQVIGDESRCCFVSCPDVIHAAMSQILNEHCIG

General information: TITO was launched using: RESULT: Template: 4OFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1873 28852 15.40 75.33 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 15.40 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_4OFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OFZ-query.scw PDB file : Tito_Scwrl_4OFZ.pdb: