Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------RRMCILTLAEKTQNPEKPNFLEDTYQEVEAPAAAMLSYGGHEIHFELDKN-----CWPRLRHGSAVPPGYSAHIYSEGSDKY--PPTSAVIEGTHVQKLPG---------FNPKGNVRFYEKLIGLS 1IFR Chain:A ((1-113)) GSHRTSGR----VAVEEV--DEEG-KFV-----RLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPP---KFTLKAGQVVTIWAAGAGATHSPPTDLVWKA---QNTWGCGNSLRTALINSTGEEVAMRKLV---

General information: TITO was launched using: RESULT: Template: 1IFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 369 35243 95.51 391.58 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 95.51 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_1IFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IFR-query.scw PDB file : Tito_Scwrl_1IFR.pdb: