Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------MGQLTDASVVLRFGYQAIRRAG------LPTEEILTKAGVALNQVDTNARTPLSAQYAFWTAAQEVSKDPDIGLHLGEHLPLYRGQVIEHLFNSSETFGEGLKRALAYQRLISDAFDAKLVVEDGRCYLTNGEQVGADNLV-----------------NRHFSECAI--SGVLRFFKFITEGQFHPIFIDFNFSEGASEDEYFRVYGCPVSLGQK----ETRLYFDPAILDFQLWQAEPELLQLHEQLAIE-KLQELARYDLVGEVR---------RAIGSTLESGETTLETVAAQLNITPRRLRTQLSEANTSFQQILSDYRCRLAKKLLANTNESVERIVYLTGFSEPSTFYRAFKRWTNETPVEYRKRKQHR 4H2Q Chain:A ((10-379)) KTRRAEVAGGGFAGLTAAIALKQNGWDVRLHEKSSELRAFGAGIYLWHNGLRVLEGLG-ALDDVLQGSHTPPT--YETWMHNKSVSKETFNGLP-------------WRIMTRSHLHDALVNRARALGVDISVNSEAVAADPVGRLTLQTGEVLEADLIVGADGVGSKVRDSIGFKQDRWVSKDGLIRLIVPRMKKELGHGEWDNTIDMWNFWPRVQRILYSPCNENELYLGLMAPAADPRGSSVP--IDLEVWV---EMFPFLEPCLIEAAKLKTARYDKYETTKLDSWTRGKVALVGDAAHAMCPALAQGAGCAMVNAFSLSQDLEE-GSSVEDALVAWETRI-RPITDRCQALSGDYAANRSLSKGNMFTPAALEAARYDPLRRVYSWPQ-

General information: TITO was launched using: RESULT: Template: 4H2Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1852 54054 29.19 173.25 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 29.19 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_4H2Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H2Q-query.scw PDB file : Tito_Scwrl_4H2Q.pdb: