Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MGIRITGTGLFHPTEIISNEELADSLNAYVEQYNQENAEKIAAGELEELRGSSAEFIEKASGIKRRYVIEKSGILDPTRLRPRLSERSNDELSIQAEWGVIAAKQAMENAGVTAEDIDVVILACSNMQRAYPAVAIEIQSALGIQ-GYAYDMNVACSAATFGLKQAADAIRSG-ARRVLLVNVEITSGHLDY-RNRDCHFIFGDVATASIIEETTTK-TGFEILDIHLFTQFSNNIRNNFGFLNRSEDAVVDDKLFRQDGRKVFKDVCPLVAKIINAQLEKMQLTANDIKRFWLHQANANMNELILKY--VAGKDADLSRTPIILDEFANTSSAGVIIALHRTGHEVDDG------EYGVISSFGAGYSVGSIVVQKHVA-- 1MZJ Chain:A ((3-336)) GLRVPERRFSRVLGVGSYRPRREVSNKEVCTWI---------------D---------STEEWIETRTGIRSRRIAEP--------------------DETIQVMGVAASRRALEHAGVDPAEIDLVVVSTMTNFVHTPPLSVAIAHELGADNAGGFDLSAACAGFCHALSIAADAVESGGSRHVLVVATERMTDVIDLA-DRSLSFLFGDGAGAAVVGPS-D-VPGIGPVVRGIDGTGLGSLHMSSSWDQYVEDPSVGRPALVMDGKRVFRWAVADVVPAAREALEVAGLTVGDLVAFVPHQANLRIIDVLVDRLGVPEHVVVS----RDAEDTGNTSSASVALALDRLVRS----GAVPGGGPALMIGFGAGLSYAGQALLLPDPPS

General information: TITO was launched using: RESULT: Template: 1MZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1822 -36993 -20.30 -118.19 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -20.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1MZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MZJ-query.scw PDB file : Tito_Scwrl_1MZJ.pdb: