Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------MQEVYCVMIEKSINPHDY----QHIHSPASVMPKNYHAKHR-IDLHQHERGQLIYATSG-----IMELSTEEELWLIPPRFGVWIPAYTPHQMTTKT---DVHLNTLYIDTYRWSKPFPDKPCGVTVSGLLHELLIRAASFPIEYPLHSVEWKTMDLLVEELKWTKGISLMTPE-PYMDKRLQDLCNILIINPADNHSLEEWSSQVGATTRTLSRLFKQEMNTTFTLWRQQLRIVHAIPSLMAGK-PISDISQELGY-SSQSTFTVMFKKIMGKTPTEYIAWANGNPTTFD 5CHH Chain:A ((4-328)) ENIQYDFEWRPLNNYLAVQRITPPRLPSEALYEWIVLNGRSPLEGLSLGEHYRLSDYGVAGLALQSAGTVGEALQLIKTNMLLFRKDIRGIAVRRSSCDTVDVDIDLQDKPDWPQSARLYHANVLASAAYAVFRDLLLGELELVRLRLPERNGDVRAYEEYFRVPVYFAGAGITFTLPEELLEAEIATANSAVFQASLALGS-KAFNTRVTREMGGYRQRIVALLEV---LQDRYPSIAWVARQLKVTERTLRRRLADE-GTNYREVLDLVRHDRARQLLRDERLRIEEVAERLGYMD-TSSFRHAFRRWTGQCANDYRQA---------

General information: TITO was launched using: RESULT: Template: 5CHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1177 20532 17.44 78.97 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 17.44 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_5CHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CHH-query.scw PDB file : Tito_Scwrl_5CHH.pdb: