Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYQMITREQCLYWDQEDELKKFKDEFALPE------GVIYLDGNSLGARPKKSLAVAQHIISQEWGE-DLINSW-NKADWWGLPTRLGDKVAKLVGAEQ-GEVVISDSTTLNLFKVLSAAVKIQADKFPEHKIIVAEKDAFPTDIYIIEGFIDLIQKGYQVELID-------GVEDLSRALEKDVAVVVLSHVNYRTGYFYDMASINEQIHS-KDALVIWDLCHSVGAVPMHLNQTDSDFAIGCTYKYLNGGPGSPALLWVNEKHRDQFWQPLSGWWSHKKPFDMAQHYEPANSIRRYLCGTQPVISMSLIECGVDIFLHADMQKIREKSLKLTDLFIQLVHQECSEFGFELITPLDHKHRGSHVSYRHEF---GYEIIQALIARGVIGDYREPAVLRFGITPLYLGFEDIWNAVQHLKATMLNSEWKNKEYLVRGEVT 5VPR Chain:A ((9-413)) ----------------MFDIQEIRSQFPILQEKVNGKDLVYLDNAATSQKPKMVLDAIN-NYYEHYNANVHRGIHTLSQVATEMMEDARKKVQRFINAKHDYEVLFTKGTTEGINLVAYAMTDL----IKKDDEIIISY----SNIVPWQMLCQRTGA--KLRVIPMNEDGTLQIDVLDEWLSEKTKLVSVNQVSNALGIVNPIDEIIRKVRAKSNAFIFIDGAQAAPHFEIDVQTMDCDFFAFSGHKM-YGPTGT-GILYGKASVLEQLN-----------------------LPFRFEAGTPNIAGNIAIGTAVDFMEKVGRSNIAAHEHALLEYAQRKLLEI---EGLKVYGEKA--NRAGVVSFNLSGIGIASDVGMILDKLGIAVRT---GTVRASFAV-YNTFEEIDILTEGVKKAQKML--------------

General information: TITO was launched using: RESULT: Template: 5VPR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1861 -11335 -6.09 -32.86 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -6.09 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_5VPR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VPR-query.scw PDB file : Tito_Scwrl_5VPR.pdb: