Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANLKEIRAKVASIKSTQKITRAMQMVAASKMRRAQERMAQGRPYADNMRRVIAHLVQANPEYKHRYMVDRPVKRVGYIIVSSDRGLAGGLNINLFKKVVQHVKAQQEQSIEVQFALIGQKAVSFFK--N-YGGKVLGATTQI-GDAPSLEQLTGSVQVMLDAFDKGELDRIYLVSNGFVNAMTQKPKVEQLVPLAPAEEGDDLNRTYGWDYIYEPEAEELLNGLLVRYIESMVYQGVIENVACEQSARMVAMKAATDNAGQLIKDLQLIYNKLRQAAITQEISEIVGGAAAV 3ZIA Chain:G ((1-276)) -ATLKEVEMRLKSIKNIEKITKTMKIVASTRLSKAEKAKISAKKMDEAEQLFYKNAETKN--------------KELIVAITSDKGLCGSIHSQLAKAVRRHLND----QPNADIVTIGDKIKMQLLRTHPNNIKL--SINGIGKDAPTFQESALIADKLLSVMKAGTYPKISIFYNDPVSSLSFEPSEKPIFNAKTIEQS-----PSFGKFEIDT-DANVPRDLFEYTLANQMLTAMAQGYAAEISARRNAMDNASKNAGDMINRYSILYNRTRQAVITNELVDIITGASS-

General information: TITO was launched using: RESULT: Template: 3ZIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1132 -35478 -31.34 -135.93 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain G : 0.77 3D Compatibility (PKB) : -31.34 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3ZIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZIA-query.scw PDB file : Tito_Scwrl_3ZIA.pdb: