Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKFRIDLLRHGESQY--SHTLRGHLDDELTAKGWQQMQATIEQVSN--QPWDVIVSSSLKRCVCFAEQLAKTAEL---PLLVNHDLKEMYFGEWEGISTQQIYETSPEL-LANFWQK------------------------PSQYCPPRAETLDQFQTRVLKGFQDLLEHMQKQNLQHALVVTHGGVIKLLACLARQQPLDDLLKMPAELGKLYSLEFSETDSQLTFKLR 4EMB Chain:A ((26-249)) -FMYKLVLVRHGESEWNKENLFTGWTDVKLSDKGIDEAVEAGLLLKQEGYSFDIAFSSLLSRANDTLNIILRELGQSYISVKKTWRLNERHYGALQGLNKSETAAKYGEDKVLIWRRSYDVPPMSLDESDDRHPIKDPRYKHIPKRELPSTECLKDTVARVIPYWTDEIAKEV-LEGKKVIVAAHGNSLRALVKYFDNLSEEDVLKLNIPTGIPLVYELDKDLNPI-----

General information: TITO was launched using: RESULT: Template: 4EMB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 943 16840 17.86 87.71 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 17.86 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4EMB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EMB-query.scw PDB file : Tito_Scwrl_4EMB.pdb: