Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLAQLVREEMLHYEQVLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDVLVIGAFVEARSCERFYALAPCVDDELGRYYRYLLKSESRHFEDYLA-LALDVAKTAKMKDPEEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA 3EZ0 Chain:B ((15-169)) -------------------------------FVADLFGMMAYGELSAFERFSADARYSPT-LHDRAVLGRIAVVEFRHYELVSARLEAMGIDAEDA-MLPFQAAVDYFHSRTRPADWYESLMKAYVIDTVSADFYRAISRYVDAGTRDVIEQIQ-----TTEVLRERLRSAL-------ADDPRLASRLALWG----------------------

General information: TITO was launched using: RESULT: Template: 3EZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 518 -61599 -118.92 -419.04 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -118.92 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3EZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZ0-query.scw PDB file : Tito_Scwrl_3EZ0.pdb: