Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTAFLFSGQGAQYQGMGKDLYEE-AVVKQTFDEASEILGYDMAELCFT-ENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALSFTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEAS-KYGIVSPANYNTPQQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFSEPSFPIISNTTTE-IMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVLSGFMKKIDKTIPVLRVENKQTFDETIAIL 3IM9 Chain:A ((11-308)) -HMAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKN--LNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLED--------

General information: TITO was launched using: RESULT: Template: 3IM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1763 6320 3.58 21.50 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 3.58 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_3IM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IM9-query.scw PDB file : Tito_Scwrl_3IM9.pdb: