Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MNDYIEKVLSGKLIAPKK------IIQACERHISDLERSKSDSFP-----YVFDEEQATKAI---KFIELL-PST--DGKAIKMLGFQK--------FILGSLYGWRT---KEGNYR---------RFNRAFTSMSRKNGKTYIASGMAANA--LIMEQEPAEARQVLFVSNALKQAKL---GYDMLSNSLRNVVKSSKFLRPQLKIMNSKIQHLPSNSF--------AMALASET---STLDGFAPTTAILDEWHEAKTRKTYNVI------KSGMTQQK-----NGLLCVIST---------AGLDLNVPMYEEYLLL----DRVLKGEE----------QADRYFIAIWELDDP-----EEIHDQEKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSY------MIAED-------------WQATEVEPQDITGKPVYIGVDLSKTDDLT-SVSWIVPLDNGELYCDSHSFVATKYGLQDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVF----QFIQDLIQ------ENDLECMGICYDPYNANSLISKAEKANY----------------PMLEV-----RQGTITLNVPTRTFREQVYEGNVIHNKNTI-----LTHAVNN--AILKTDNNGIQINKSKNSNKIDPIAALINAYVFAM----------------------------------------DYFTTTEGAKADNEFYTSEEFSF------------------------- 5C2V Chain:A ((1-781)) EPVMTGGPVQGKALWTDYSGMSKEVQGPVSQILFTQSPRTAKGDPYQNYPHYIPEGSRIVLFDLNTKELKVLTNDFATAFDPCTYWDGKKFAFAGVHKKGGGCQIWEMNIDGSGLRQMTDLKGTCRSPIYYAAGSIEEGEGRIIWRDREGDWKEHGMVEKTGMIIFSGSPEGVMDEFHNPYAYNLYRLDTQGGKIIQRITGHVLSGIEF--PHLNTTIDQITYNLSSNFDPWLTPDGNILFSSVQANGSRAGGEGRVMICVDNWDGAYPRPIYGNCDGEIGGTSGRSQAKITFGDRKIVYVESPYMNWGVGQLAAVSWDAPFNKTYEKLTGKDGGLYRSPYPLPDDRMLVSYAERGDFGIYWFNFSKCAAGDKVYDDPNWNDHQPAPVYVKYKPRWINTFTAGKNFGVT--------VVTYQPFDQVKVEGYPHSWGTWICFDTTLSDQPVGPYPHQKAKNVSHGDIKAVRIIQGYQCVEPDSTRFRVGAGAHLLGGER---SSSNSGTAF-----QQRGIIGYQYVESDGSTVTSQL-SDVPYYMQILDDKGMSVQTALTWAYLRPYHGRICSGCHYGSYRGRAFKNIHAKALYNWWYDDRSHYDSPFAFRYLKFDNDGNYKGVKHGEDVVGTTSQPV-EGLTLDKQRTVDFRRDIQPILDAKCAMCHDSNNPPNLGGGLELVSVDGIAAYSRAYNSLLEPQRGKDPNIGGKYVNPSAAINSLLVWRLYEAELSANAPREKIFPIEGRLLHNKFLTQDERYAIVEWIDLGAQWDNIPGPDFYPGYLV

General information: TITO was launched using: RESULT: Template: 5C2V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3311 125017 37.76 229.81 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 37.76 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.055

(partial model without unconserved sides chains): PDB file : Tito_5C2V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C2V-query.scw PDB file : Tito_Scwrl_5C2V.pdb: