Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSRLPEIMSWEVETVRGVTVSIGRWR 5ARM Chain:A ((1-130)) MHVEAMISKHPQATD-----------RSLVQCVEMCFDCAQTCAACADACL----------------GEDKVADLRHCIRLNLDCAEICVAAGSIASRAAGTEESILRTMLQTCAEMCRMCEEECRR--------HAGNHEHCRICADVCKECET-ACRSATG---------

General information: TITO was launched using: RESULT: Template: 5ARM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 563 -52772 -93.73 -415.52 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -93.73 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.165

(partial model without unconserved sides chains): PDB file : Tito_5ARM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ARM-query.scw PDB file : Tito_Scwrl_5ARM.pdb: