Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQT-----ET--ESILGLGGK--VLVQLIQA-QE-SGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGADHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEPFILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVP-SASWIAAGDY-HHHLAVNEWRGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWM----TSSKLEITDSDGIVTRIRLAR 1ZSW Chain:A ((22-298)) -SNAMYEIKGHHHISMVTKNANENNHFYKNVLGLRRVKMTVNQDDPSMYHLFYGDKTGSPGTELSFFEIPLVGRTYRGTNAITRIGLLVPSEDSLHYWKERFEKFDVKHSEMTTYANRPALQFEDAEGLRLVLLVSNGEKVEHWE----------TWEKSEV---------PAKHQIQGMGSVELTVRRLDKMASTLTEIFGYTEVSRNDQEAIFQSIKGEAFGEIVVKYLDGPT---EKPGRGSIHHLAIRVKNDAELAYWEEQVKQRGFHSSGIIDRFYFKSLYFRESNGILFEIATD-

General information: TITO was launched using: RESULT: Template: 1ZSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1381 46161 33.43 177.54 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 33.43 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_1ZSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZSW-query.scw PDB file : Tito_Scwrl_1ZSW.pdb: