Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSK-PWQTLWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDLGLVFPESLKEFES----EDSFAKEISAEEANKINDADVIITYGDD----KTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK 3EIW Chain:A ((5-296)) ------------------------------------ASTISVKDENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKK-------KRIIKPVREKIGDYTSVGTR-KQPNLEEISKLKPDLIIADSSR-HKGINKELNKIAPTLSLKSFDGDYKQNINSFKTIAKALNKEKEGEKRLAEHDKLINKYKDEIKFDR----NQKVLPAVVA---KAGLLAHPNYSYVGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQEDATWKKLNAVKNNRVDIVDRD--VW-ARSRGLISSEEMAKELVELSKKEQK---

General information: TITO was launched using: RESULT: Template: 3EIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1574 -2649 -1.68 -9.36 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -1.68 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_3EIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EIW-query.scw PDB file : Tito_Scwrl_3EIW.pdb: