Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIPIKIIQ-----ASKSDLPEIGALQTSSF---------PAEKQQLSHILEESIRKCADTFLLARDENQLLGYILSSPQS-----DNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAVELD-YKGIRLESPDE----LLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ------- 1BO4 Chain:A ((25-160)) ---GIIRTCRLGPD-QVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLR---SKTFIALAAFDQE-AVVGALAAYVL-PKFEQ-PRSEIYIYDLAVSGEHRRQGIATALINLLKHEANAL-GAYVIYVQADYGDDPA--------------------------------------VALYTKL

General information: TITO was launched using: RESULT: Template: 1BO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 420 -6563 -15.63 -62.50 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -15.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1BO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BO4-query.scw PDB file : Tito_Scwrl_1BO4.pdb: