Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTI---DADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDLDQKQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFKQELYLIDFEKTRLISSPDPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLTQTRLQANSYHEWAVLHCQKLNVELPSSFQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 4Y0X Chain:A ((115-364)) -----------------DIVAGQYEVKGCIAHGGLGW-IYLALDRNVNGRPVVLKGLVHSGDAEAQAMAMAERQFLAEVVH--PSIVQIFNFVEHTDR---HGDPVGYIVMEYVGGQSLKRSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINS-FGYLYGTPGFQAPEIVRTGP-TVATDIYTVGRTLAALTLDLPTRNGRYVDG--LPEDDPVL---KTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQLTG---

General information: TITO was launched using: RESULT: Template: 4Y0X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1179 10303 8.74 42.05 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 8.74 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_4Y0X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y0X-query.scw PDB file : Tito_Scwrl_4Y0X.pdb: