Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITHSLKDKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSSETQADAEQTLADVKALGADAILIQADLTDIDNIEKLFLDTKQHFGGIDIAINTVGKVLKKPILETTEQEFDSMSDINSKIAYFFIQSAGRHLNNGGKICSIVTSLLAAYT-GFYSTYEGLKAPVEHYTRTASKEFGDRQISVTAVAPGPMDTPFF-------------YGQESAEAVAYHKSASALGGLTKIEDIEPLVRFLVT-DGWWITGQTIFANGGYTTR 3QWF Chain:D ((16-268)) -----LDGKVALVTGSGRGIGAAVAVHLGRLGAKVVVNYANSTK--DAEKVVSEIKALGSDAIAIKADIRQVPEIVKLFDQAVAHFGHLDIAVSNSGVVSFGHLKDVTEEEFDRVFSLNTRGQFFVAREAYRHLTEGGRIVLTSSNTSKDFSVPKHSLYSGSKGAVDSFVRIFSKDCGDKKITVNAVAPGGTVTDMFHEVSHHYIPNGTSYTAEQRQQMAAHASPLHRNGWP--QDVANVVGFLVSKEGEWVNGKVLTLDGG----

General information: TITO was launched using: RESULT: Template: 3QWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1329 -59235 -44.57 -248.88 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -44.57 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3QWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QWF-query.scw PDB file : Tito_Scwrl_3QWF.pdb: