Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSLMSVTEKETSNSLYRQWQILSRLPTGKWIGTRELQEMLEREGIE-ISLRTIQRDLNQIAQRFPIESNKAVPQGWRWQSDAPIQSLPHMTSSQAVTFMMVEEHLKRLLPPSLLDEMGPWFDLAKRSL-----STQNNVRQWINRVRIVPASQPLIPPVVDRAAQQAIYEGL--------LQDKQIECVYRARVNQGEDKTYILNPLALVQKG--------AVIYLICTRHDKTEVQTFALHRFKSAKVLES-RALHPVNFDIDHYI--------------------DSGALG--------------FRVDYNQPTESIQL---TLTMTEQTAKTFYESQLSKDQTITPIEENIVEVTATVPFTSQLVWWLRSFGKKLLHIEPVQVHNAVREIEPDSK-- 5IWZ Chain:A ((4-397)) ---------MPVRPDLQQLEKCIDDALRKN--DFKPLLALLQIDICEDVKIKCSKQFLRKLDDLICRELNK----KDIQTVSSILISIGRCSKN---IFILGQAGLQTMIKQGLVQKMVSWFENSKEIILNQDEAVMNMIEDLFDLLMVIYDISDEGKNQVLESFIPQICALVIDSRVNFCIQQEALKKMNLMLDRINQEMLTLMSNMGERILDVGDYELQVGIVEALCRMTTEKRRQELAYEWFSMDFIANAFKEIKDCEFETDCRIFLNLVNGILGDKRRVYTFPCLSAFLGKYELQIPSDEKLEEFWIDFNLGSHTLSFYIEAVTVPEEKVQMYNIEVRESKKLLTLTLKNIVKISK--KEGKELLFYFD---------ESLEITNVTKKVFGGLEHH

General information: TITO was launched using: RESULT: Template: 5IWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1385 -8380 -6.05 -27.03 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.05 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_5IWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IWZ-query.scw PDB file : Tito_Scwrl_5IWZ.pdb: