Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MLNSEFQPNWLYPPSEVI----KNFIQLN-NIDTSAFDVSTKKIIDD------------LVNQNTKIDLYTS---------EVLKEAIG--------------GTTQFWLNIQNQFTINSLRIQNSSVDNNFS------EYRNLVNELKKL-----HWTPE-------SKYDYLDQINLKSFFGISEFSL-------LTKKYIV----------QHNLRV--------KFKNIGQY-------NSSELNVATLIRKAE-------LEAKKQPILSPWNKLLFIEKLQE----IKKLSKVKGFKNFRNE--------LLSICNQCGVAFIVLDTLNK---SPIRGISKFLDSNIGLIIVTTKYNKDDVFWQTFFHEAGHLVLHSKEMVFSDQ--GDLNKEASHNLE--------EAQADEFMLTHILHPYTIEEVTNMFN--TRIMYK--SKTESWRNICQIARQVNISPSLLTGILKFQGIIPYSYF-TNGHEKLYD 2I3O Chain:D ((2-516)) FRSRPNALSQRSVIASSSELASLAGRDILKRGGNIFDAALAVSALCVTQNNLCGLGGDLFALIRDENGQIDLNGSGQASRAVSIDYYESGLTKIPERGPYAAITVPGIAGSWDEIFRKFATDIADILEPAIRTASAGFPITQNYSDSIARSAPVIGQYRGWSSIFPNGSVPVAGEILKQPDLAESFRLSEEGFRSFYDGSLADIIIAGLEGTGSPLSDRDLRVYRPLIGKPVFTDLDEFRIYETSPNSQGITVIEWIRGESHGYDSRTWEAKIEDIFETEEAYDKRRKITDPSYNIAQHDSANGKGLPKRDHNDIGDTTYFSISDSEGRSVSIIQSNYGFGSGIVPKGTGFVLQNRGSYFTLQRDHPNALPGKRTFHTLAACVEKEHDLYASLGSGGDIQPQVQQILEILKDNTDPQAILDK---PRWTEPYTIYEAPGAVYVESEELYRNVSKQISGRKVVLRDVSQEFGTAQITTLIRGDVVVGAADPRGDGIAIPYS

General information: TITO was launched using: RESULT: Template: 2I3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1941 74687 38.48 203.51 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : 38.48 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.044

(partial model without unconserved sides chains): PDB file : Tito_2I3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I3O-query.scw PDB file : Tito_Scwrl_2I3O.pdb: