Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLYATQDEKKQAAAKAALKH----LPKGGILGVGTGSTVNFLIDLLPELQ--LEAAVASSQATADRLKKLGIEVVDMNHVVSLDAYVDGADEIDRHMHMIKGGGAALTREKIVASIAKKFVCIVDDSKWVDQLGRDFPLPVEVIPMARSAVARKLVSLGGDPVYRE-----GVVTDNGNVILDVFNLNILNAIDLEKTINNIPGVVTNGIFALNPATIAIVATNDGIEERTAQ 3UW1 Chain:A ((7-235)) --LLMTQDELKRLVGEAAARYVTDNVPQGAVIGVGTGSTANCFIDALAAVKDRYRGAVSSSVATTERLKSHGIRVFDLNEIESLQVYVDGADEIDESGAMIKGGGGALTREKIVASVAETFVCIADASKRVAMLG-QFPLPVEVVPMARTAIGRRLAALGGVPVLRVKQDGTPYVTDNGNEILDVKGLRIDDPRALEAAINGWPGVVTVGLFAQRGADLCLLGTEHGVETLR--

General information: TITO was launched using: RESULT: Template: 3UW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1235 428 0.35 1.96 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : 0.35 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3UW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UW1-query.scw PDB file : Tito_Scwrl_3UW1.pdb: