Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAQYKVLCVCLGNICRSPTAEVVFRHYCDQHQLN---IIVDSARTSNYHPNKAPDQRSQLHAKKRGYDLSSLRARQLSTQDFLDFDLILAMDHQNFDDIHDLLQRAIFQFGSHQIRAKVALMSEHDPQ--YPQQALPDPYYGGDEGFERVLDQCESSALAWINILKKQLNV 1D2A Chain:B ((9-147)) ------VAFIALGNFCRSPMAEAIFKHEVEKANLENRFNKIDSFGTSNYHVGESPDHRTVSICKQHGVKINH-KGKQIKTKHFDEYDYIIGMDESNINNL-----KKIQPEGS---KAKVCLFGDWNTNDGTVQTIIEDPWYGDIQDFEYNFKQ------------------

General information: TITO was launched using: RESULT: Template: 1D2A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 624 13402 21.48 100.01 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 21.48 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_1D2A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D2A-query.scw PDB file : Tito_Scwrl_1D2A.pdb: