Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATALTLACSLLSVMSISQAQAADNIDVSFQTILQQERNWAGLQSKSLKVGDITWSYSEGGSSTKPTLLLIHGLAGSRDNWNRVAHYLTTNYHVIIPDLPGSGETIVSHDFDYSVPNLAEKLRRFVEAANLKGPIHIAGHSLGGSIALLYAGQYPFETKSLFLVDSGGIFRSANTIYLKDLT-YLKQLLVSKKGDFNYLLKQTMFNP--PFIPREFLQAQEKLMINQAPQTQKLVDQLIALNKVYTPDSFAVLTKTIDAPTLILWGKQDKIINVEVANELKRLLKNAQPPVILENVGHMPILEAEQLVIQQYVPFLLKVETNQSSKTTTP 5W15 Chain:D ((16-275)) --------------------------------------------------FNFEGHRIAWGTLGE--GPPLVLVHGTPFSSQVWRRIAPWLARRHRVFFYDLLGYGQSDMPD-ADVSLGRQNVLFGALLDEWK-ISRPRVLAHDYGGATVLRAHFLDGIAYSDLTLVNPVAIAPQGSP-FVRHVAQHEAAFTGLPAYAHHALVSAYIGQAVAQPLSDDVLSIYRAPWLT-PAGQAAFYRQIAQMRQRYI-EDAEARYAPPDFPVRIVWGEDDRWIPLEQGQALADRIAN-GKLIRVPRAGHLVQEDAPEAIVAAVLDR---------------

General information: TITO was launched using: RESULT: Template: 5W15.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1489 -85941 -57.72 -334.40 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -57.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_5W15.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5W15-query.scw PDB file : Tito_Scwrl_5W15.pdb: