Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFKVKNV---DNYAGHI-DL-G-EGDETLGIFGHMDVVPAGD---GWD----TDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESG-----WGDMAYYFEH---------EEEPDFGFSPDAEFP-IINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKG-AHGA------SPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEE----NGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTR-GARVMEPHYVPMDDPLVATLLQVYEDHTGEKGYEQ---IIGGGTYGRLLK----RGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 5K8P Chain:C ((7-422)) -VAKVMKTLDGMREGLIQTAVELGSIEAPTGR------------EGAAGDYVYEWMARNGFGPERVGVFDDRFNVVGRLRGTGGGASLSFNSHLDTIMAREDTARFADANDRIYHEAWHEEGRIYGYSVVNCKGPMACWLIAAKALKEAGAALKGDVVLTAVCGEIDCEPVDEFQGHDYLAEDIGARYAISHGAISDYALVAEATNFKPAWV---------------------------------------------------------------------EAGKVFLKVTVFAGPSRYTPYVPRPVAALDSPNAIVRMAKLVEALEEW------ADNYEK----RYT------REYGGGTVVPKVAIGAIRGGVPYKIYAFPELCSIYMDIRLNPDTNPLVVQREVEAVVSKLGLKAEVKPFLFRRGYEAQGIEPLQNALEVAHREVVGRPTERPGSPECSMWRDTNPYNELGIPSLTYGCGG-----GAGGGNTYFLVDDMLKAAKVYAMTAMDLCN

General information: TITO was launched using: RESULT: Template: 5K8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1901 13887 7.31 37.63 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 7.31 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_5K8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8P-query.scw PDB file : Tito_Scwrl_5K8P.pdb: