Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKMTRRSTMLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTNDLECI---TDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFF--NVLGHYLSGEHNWSNIAD-----------------TTFISLSKVYNLRNELINF--FKQMQYLNADGHFEIPEKDYRSLLLTIIYETNRTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFSQEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKD-LNVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKTYLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRKLTISLSLLNEHKRKSPIE------VFIVAPSDFRYLYYRQQLEDI---LGEHFSISNIICKQL---REIVDDTFFCTQR-----IILCDSSLYQEGLGSEKTIIYPITFQTIHTVIDQLNKQI 4TQT Chain:A ((14-490)) ASKVIKGGTVITA-------DRTFRADILIEDG--KIAAIGDSLEGDEVIDASGCYVMPGGIDPHTHLQMPFMGTYSSDD--FDTG-TAAALAGGTTMVVDFVLPDSEGNLLDALQEWFQKAGKARTDYSFHMAITGWNERTFNEMAEVVKRGINTFHFMAYKGALMVNDDEMFASFQRCAELGAMPLVHAENGDIVAQLQAKLMAEGN---DGPEAHAYSRP-PEVEGEATNRAIMIADQAG--VPLYVVHV-SCEQSHEAIRRARQKGMRVFG--------EPLIQHLTLDESEYHNRDWDYAARRVMSPPFRDKLNQDSLWAGLAAG---SLQCVATDHCAFTTEQKRYGIGNFTKI----PNGTGGLEERMPVLWTRG---VRTGRLTPNEFVAVTSTNIAKI------LNIYPQKGAVVPGADADLVIWDPETTKKISAKTQHSSIDYNVFEGFELKGLPIMTLSRGRIAFDKGQVTAKPGDGRFIEREPNGAVNRALSQWKEIVAP----------RKVERSA

General information: TITO was launched using: RESULT: Template: 4TQT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2772 20855 7.52 48.05 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 7.52 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.136

(partial model without unconserved sides chains): PDB file : Tito_4TQT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQT-query.scw PDB file : Tito_Scwrl_4TQT.pdb: