Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMNKSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSLIDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKK-ENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYGFKQIVE 1JI0 Chain:A ((135-236)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLGGTLSGGEQQMLAIGRALMSRPKLLMMDEPSLGLAPILVS-EVFEVIQKINQEGTTILLVEQNALGA-LKVAHYGYVLETGQ---IVLEGKASELLDNEMVRKAY---------------------

General information: TITO was launched using: RESULT: Template: 1JI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 -23011 -70.37 -227.83 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -70.37 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.289

(partial model without unconserved sides chains): PDB file : Tito_1JI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JI0-query.scw PDB file : Tito_Scwrl_1JI0.pdb: