Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGH-HYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKG-MKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN-------QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKRF-------STGNRYISFNVY--------- 5L3X Chain:B ((1-405)) ------GEITSVSTACQQLEVFSRVLRTSLATILDGGE----ENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHILAYAASVVETW-----KKNKRVSINKDELKSTSKAVETVHNLCCNENASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQL------------QPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSALDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIADPVTEFIAHCKSNFIMVN

General information: TITO was launched using: RESULT: Template: 5L3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1442 -35108 -24.35 -96.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -24.35 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_5L3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3X-query.scw PDB file : Tito_Scwrl_5L3X.pdb: