Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEE------VEEMMNPERIAVAAV-DQDELVGFIGAIP---QYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGI-TIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANGW-DKPDIWMAKTIIPRPNSQ- 1B87 Chain:A ((1-181)) MIISEFDRN--------NPVLKDQLSDLLRLTW--PEEYGDSSAEEVEEMMNPERIAVAAVDQ-DELVGFIGAIPQYG---ITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR-GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANG-WDKPDIWMAKTIIP--RPDS

General information: TITO was launched using: RESULT: Template: 1B87.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 669 9393 14.04 57.27 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : 14.04 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_1B87.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B87-query.scw PDB file : Tito_Scwrl_1B87.pdb: