Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAVEQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLER-INLSDHVIITDVSSTKQQVVTSAEQ---KNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTK-KDVEKLQELFCGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDITRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKESRDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGILQISFKNQQDLLQAKRIIMSNTSYHCWIK 5USC Chain:A ((17-379)) -RKKVVLIGTGLIGGSLALAIKKD-HDVTITGYDIFQEQVERAKELHVVDEIAVDLQHACEEAHLIVFASPVEETKKLLHKLASFHL-REDVIVTDVGSTKGSIMNEAEALFSKEISFIGGHPMAGSHKTGVESAKAHLFENAFYILTPMHHVPNEHVEELKDWLKGTGSHFLVLNTEEHDYVTGIVSHFPHLIAAGLVKQVEKHAGDNPLIHQLAAGGFKDITRIASSSPKMWSDIVKQNREHLMVLLKEWISEMEDLYDTVSSGDAGEIQNYFADAKEYRDSLPVRKRGAIPAYHDLYVDVLDKVGALAHVTSILAREEISITNLQILEAREGLLGVLRISFQREEDRMKAKLALGEEK-YQTYE-

General information: TITO was launched using: RESULT: Template: 5USC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1543 -52227 -33.85 -145.88 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -33.85 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_5USC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5USC-query.scw PDB file : Tito_Scwrl_5USC.pdb: