Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------MAHKHKLDSILDFPEASEREDNIIELKTWMS-------RLRCNKDDQIKSNSVVNAE------LILTNDSNLA---GTIAYNEFSG---YIHLLKDSPWINRTAGEWEDSFEDALTAYIEENYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPR-------------LETLFIDLLGVDDNLYTRE-VTKRWIVGSVARIYKPGI-KFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGEKKDDYMQLQGNVIIELG---ELSSMNKTKIEQIKNFISAKIDKI---RPPYGRNVIAWARQCVFIGTSNDGQYLKD----------DTGNRRFYPLPC----KNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIYFSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYPDSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIALIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK-------------------------------------------------------------------------------------------- 4Z24 Chain:A ((4-652)) NLTGDIVIIGAGAAGSLLAHYLARFSNMKIILLEAGHSHFNDPVVTDPMGFFGKYNPPNENISMSQNPSYSWQGAQEPNTGAYGNRPIIAHGMGFGGSTMINRLNLVVGGRTVFDNDWPVGWKYDDVKNYFRRVLVDINPVRDNTKASITSVALDALRIIAEQQIASGEPVDFLLNKATGNVP-NVEKTTPDAVPLNLNDYEGVNSVVAFSSFYMGVNQLSDGNYIRKYAGNTYLNRNYVDENGRGIGKFSGLRVVSD------AVVDRIIFKGNRAVGVNYIDREGIMHYVKVNKEVVVTSGAFYTPTILQRSGIGDFTYLSSIGVKNLVYNNPLVGTGLKNHYSPVTITRVHGEPSEVSRFLSNMAANPTNMGFKGLAELGFHRLDPNKPANANTVTYRKYQLMMTAGVGIPAEQQYLSGLSPSSNNLFTLIADD----------IRFAPEGYIKIGTPNIPRDVPKIFFNTFVTYTPTSAPADQQWPIAQKTL-----APLISALLGYDIIYQTLMSMNQTARDSGFQVSLEMVYPLNDLIYKLHNGLATYGANWWHYFVPTLVGDDTPAGREFADTLSKLSYYPRVGAHLDSHQGCSCSIGRTVDSNLKVIGTQNVRVADLSAAAFPPGGNTWATASMIGARAVDLILGFPYLRDLPVNDVPILNVN

General information: TITO was launched using: RESULT: Template: 4Z24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2492 80143 32.16 170.88 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 32.16 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.055

(partial model without unconserved sides chains): PDB file : Tito_4Z24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z24-query.scw PDB file : Tito_Scwrl_4Z24.pdb: