Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence TEKCIITIRKNDPALGKHTLYDMVSLPPDSDYTFGKFPPATAGVTIGVDDTCRAGLVVGDLNPPYDYRGMENPFYGLTGVELVRQL-HDWRENPENVINFSLW 4EYT Chain:A ((10-106)) KQNCLIKIINIPQGTLKAEVVLAVR-HLGYEFYCDYIDGQ---AMIRFQNSDEQRLAIQKLLNHNN--NKLQIEIRGQICDVISTIPEDEEKNYWNYIKFKKN

General information: TITO was launched using: RESULT: Template: 4EYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 362 -43489 -120.14 -453.01 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -120.14 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_4EYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EYT-query.scw PDB file : Tito_Scwrl_4EYT.pdb: