Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCVPDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLLGRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYMDTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIIDGVE-RL-HGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFVAKLEEMGVRMTVSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVNHVFELAKMDADISTTNGHILYTGGRDLRGASVKA-TDLRAGAALVIAGLMAEGKTEITNIEFILRGYSDIIEKLRNLGADIRLVED 5UJS Chain:A ((25-441)) MTYLEIEGTNHLSGNVTISGAKNAALPLIVSSILAKNEVKINNVPNVADIKTLISLLENLGAKVNFQNNSALLNTNTLNQTIAKYDIVRKMRASILTLGPLLARFGHCEVSLPGGCAIGQR-IDLHLLALEKMGANIQIK-QGYV-VAS-GNLKGNEILFDKITVTGSENIIMAAALAKGKTKLLNVAKEPEVVQLCEVLKDAGLEIKGIGTDELEIYGSDGELLEFKEFSVIPDRIEAGTYLCAGAITNSKITLDKVNATHLSAVLAKLHQMGFETLITEDSITLLPAKEIKPVEIMTSEYPGFPTDMQAQFMALALKANGTSIIDERL--NRFMHVSELLRMGADIKLNGHIATIVGGKELNAADV-MATDLRASSALILAALAAKGTSKVHRIYHLDRGYENLEEKFKDLGAKITRLE-

General information: TITO was launched using: RESULT: Template: 5UJS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2493 -141346 -56.70 -343.91 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -56.70 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_5UJS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UJS-query.scw PDB file : Tito_Scwrl_5UJS.pdb: