Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFEDQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAIDEIRQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTLAYGTY-GHILNLGPDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRRK--YSTSAFWKDVQKFNASAIGYVGELCRYLMDAPVTELDRNHRVTKM--IGNGMRPNIWDKFKQRFGVKEVLELYASSEGNVGFSNIFN---FDNTVGFSPTPYAIIQFDKEKNEPIRDKNGWCQKVKAGEVGLLIGKITSRSPF-DGYTDPEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNTNGRAGMAAITLADGE-ELNDADLTEMVTVFKKCLPTYAVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 4WV3 Chain:B ((9-519)) ---------------------------------------------------------------------YYNAVDILERNLPVRANKTALFTPDREMTFRQVSNEANQVGNALKGLGVRFGECVGLLTLDSAEWVTSFFGIVKLGAIAVGINTLLKPPEYEYILRDCRARVLIVHQEFLPLIESIRGNLPML-EHIVVIGEGP--------QEGYLSFNDWIRPQP--TTLEAAQSHREDICSLNYSSGTTGGPKGIPHAHKDYPLTAQLWGVNVLGLRESDRTFALAKLFFTFGTGGNLIFPWYVGASCVLFPGAARVASNVLSTISRFKPTIFYNAPTGYAAALALKDFSQHDLSSLRLCVSASEALPAALWYAWKEATG-VDIIDGIGCTENFHIFISNRPGDIRPGSSGKPVEGYELK---------LVDD--EGKTVPAGEIGNVLL--RSETAALSYWHNFEKSRQTFQ-------GEWLATGDKYFVDADGYYWHAGRSDDMLKVGGIWVSPVEVESTLIQHPAVQECAVIGCPD-----LIKPKAFIILKP-QIPSEALIRQITDHCTEKMAAYKRPRWIEFVTELPKTATGKIQRFKLRSAAKLA-----------------------------------

General information: TITO was launched using: RESULT: Template: 4WV3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3076 -178204 -57.93 -358.56 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -57.93 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_4WV3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WV3-query.scw PDB file : Tito_Scwrl_4WV3.pdb: