Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKMRLKHIILTTCLCSTLAMAELPQDSRRPGGIAVIPLTSDITQVTFQHKPVLISQEGQQRYAVFGIPLSTPLGSVQLETNKAPIQIEVKSYPYAEQRLKVTNQDYVNPNQSQLDRYAQEAKEQNDVYSSFTQSSWQALPKFIRPTSG-KFTNSFGRKRF-FNGEERAPHSGLDIPAPIGQKVVAPADGVVVQTGHYFFNGQTVLIDHGQGLISMFCHLSEIR--VEKGQHIRQGETLGLVGKTGRVTGPHLHWGMSLNNARVDPQLFLTTTH 4RNZ Chain:A ((187-319)) -------------------------------------------------------------------------------------------------------------------------------------------AGFLLETPVKYTRISSPFSYGRFH------RPHYGVDYAAKHGSLIHSASDGRVGFMGVKAGYGKVVEI-HLNELRLVYAHMSAFANGLKKGSFVKKGQIIGRVG----STGPHLHFGVYKNSRPINPLGYIRT--

General information: TITO was launched using: RESULT: Template: 4RNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 612 -9343 -15.27 -78.51 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -15.27 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4RNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNZ-query.scw PDB file : Tito_Scwrl_4RNZ.pdb: