Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMVLIKLKPNRN---FNFDQVRAGESILLTLLIDGVYQQRSYSIIEVT-TQGEIALGIKVQ--GLVSRAAQ-LLHVGECVEISQPQGDFTLHQG-QQPAILIASGSGVTAIYSLLQQALKQQL-EQIHVIYFNRA---EIFHAELKALAEQYPQLQYHFFNTTEQ------KQHLTESLLQKLV-PDFEQTATYVCGHHGMMQQANEIYTQKGAQ-SQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 1TVC Chain:A ((8-248)) -----------------------------VGSFEAEVVGLNWVSSNTVQFLLQKRPDECGNRGVKFEPGQFMDLTIPG--TDVSRSYSPANLPNPEGRLEFLIRVLPEGRFSDYLRNDARVGQVLSVKGPLGVFGLKERGMAPRYFVAGGTGLAPVVSMVRQMQEWTAPNETRIYFGVNTEPELFYIDELKSLERSMRNLTVKACVWHPSGDWEGEQGSPID-ALREDLESSDANPDIYLCGPPGMIDAACELVRSRGIPGEQVFFEKFLPSG----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TVC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1191 -15945 -13.39 -72.15 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -13.39 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_1TVC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TVC-query.scw PDB file : Tito_Scwrl_1TVC.pdb: