Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGVRGNGVIRKLAAPFKILKATFSAMRYMKQLKVDAVAGFG-GYVAGPGGLAARLLGIPVLIHEQNAVAGFTNAQLSRVAKVVCEAFPNTFPASEK---------------VVTTGNPVRREITDILSPKWRYDEREQAGKPLNILIVGGSLGAKALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLLTEMAVKARQHAQPNATQHVVGLIQKM---- 1F0K Chain:A ((7-357)) ---------KRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADRMEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGMGG-YVSGPGGLAAWSLGIPVVLHEQNGIAG------------------LTNKWLAKIATKVMQAFPGAFPNAEVVGNPVRTDVLALPL---PQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQSGKGSQQSVEQAYAE-AGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPALFVPFQHK-DRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGW-SRETLLTMAERARAASIPDATERVANEVSRVARAL

General information: TITO was launched using: RESULT: Template: 1F0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1910 -60426 -31.64 -182.55 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -31.64 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_1F0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F0K-query.scw PDB file : Tito_Scwrl_1F0K.pdb: