Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQTTVRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRGDIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYALELIEFTENSITLDVTCSKGTYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQR----------EALLLPVYAPIYHFLKIQVPEGREKYFCNGLE---SNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILNL 1K8W Chain:A ((22-324)) ----------MDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHTGALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT-FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLFIRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEHLRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAPLEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVE

General information: TITO was launched using: RESULT: Template: 1K8W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1465 -102436 -69.92 -353.23 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -69.92 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1K8W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K8W-query.scw PDB file : Tito_Scwrl_1K8W.pdb: