Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVKILAVDMDGTFLNSKKQYNKARFLKQYEQLKQNNIRFVVASGNQLAKLVTYFPEI----N-HEIAFIAENGAHV-VDAGQELAFAHLSKEQFIEILNAIDPV-YTSKMVICGKQSAYVHSSMNAEDYAKVARYFEK--L--TVIDDFYALDDLVCKITFTAQENESFTIFEHFQKQSFVKDKVLVPVSSGFNFIDLILPDQHKAHGLKLLLQKWQVQPDQVVAIGDNNNDIQMIKAAGYGFAVEA----LKSVTPYTTANNEQEGALQVIDLVLQHQPPFA 4ZEV Chain:B ((27-295)) DEIKIIFTALDGTLLNSENKVS-EQNLESLIRAQEKGIKVVIATGRSIFSVENVIGEHVKKNRISLLPGIYMNGCVTFDEKGSRVIDRIMNNDLKMEIHEFSKQINISKYAIWFCLEKTYCFEIND-----CIREYMEVEALNPDVIEDNMLEGLTVYKVLFSLPENILENTLKLCREK---FSHRINVANTFQSYVELFHQHTNKFEGVKEICKYYNISLNNALAMGDGENDIEMLSGLTHSVGVHNASEKVKNSAAYVGPSNNEHAISHVLKTFCD------

General information: TITO was launched using: RESULT: Template: 4ZEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1372 -44346 -32.32 -174.59 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -32.32 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_4ZEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZEV-query.scw PDB file : Tito_Scwrl_4ZEV.pdb: