Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTISKGVSLMKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEATGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYEIPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF 1S8A Chain:C ((14-129)) ------------IALVAHDHCKQMLMSWVERHQPLLEQHVLYATGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPQDPDVKALLRLATVWNIPVATNVATADFIIQS----------------------

General information: TITO was launched using: RESULT: Template: 1S8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 535 -102873 -192.29 -886.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -192.29 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_1S8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S8A-query.scw PDB file : Tito_Scwrl_1S8A.pdb: