Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPLH--AVDFLADYFKEKTN-TSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLKAITR-----------FYFDFHWKQER--ILGCVINDPLAVAYFIEEAICEGFKSFTAVETQG-ISRGQTLVDRYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 3G5I Chain:C ((6-300)) ---ILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLRMLTLLNRTDIPVAGGAVKPLMREL----------GL---DGPALPEPTFAPQNCTAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAM-GLGNWTPAAEFNIYVDPEAAEIVFQSGIPVV-MAGLDVTHK-----------AQIHVEDTERFRAIGNPVSTIVAELLDF--------EKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGF--------------------------

General information: TITO was launched using: RESULT: Template: 3G5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1560 -75363 -48.31 -288.75 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -48.31 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_3G5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G5I-query.scw PDB file : Tito_Scwrl_3G5I.pdb: