Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSGPLRMPTPSAPSTPPPPTATQHHHTSSWRSWGTKGTGQRVAEGGGSTTTGSPNSSAAPPSVSVTVVVLAALTAATVSVAYNSYKVRSRSSRVYRSQSTTPGHSCTRHGEASDPSHTGDIAMDSVKTASPLSPSTARSDATTCCRSTSLGGCGSTAAHTVAGENPSSTAASENELDEGGQRERDVMAQSPSLALHTDTAADAGARSGNRDMRFALCSAPPLQMAHDSTAEFHDRCATPAASAAPSPPPPETPKALLSNYYDLEPYALQGLAGGAHGWKRVFGLLKPEMRRRSGRDEACAEDGGTAADGIPGRRAPGSLTRTYDGSRVTTTDSPALLRESPGAIVPVTPAAATRSPTRRASAVESMYRSSVVKSDVSVHSRVPGQPVATGKPCTARWYGDDHKLFSVAAASSTSASTLAQPLHPPRLSVSPVAGVPSPPSRFLNSFFFPVLQHLSFCVSRSVGSGPGESTTTGEATSGTAGSGVHTASTTNTFATSAALAVAQRNLQRDISTLVATTASVRLGASASSSFPGSHAHTARCSAVSSVAVSPRAGATGSCRSQQQHEGVTAAESCSSFAALHAGRSSSGGQRRKGVSVEGEEGAAQHVLGSHARFLSAEDKQQPGMRGSPRHQSSGACRQTPASSQKSDRSATWSGAGPCKQSIRESAQERRMPPHPSPRAAATAAGATADSGAVPAPPEASSTEEPNAAGTESVGEPASPPTMAHCCTIAEQLAVLTSRREQQHRYTQQLQASRRSAGLGAGAENALPSPLATPSSPAYTGAAAASTTLDVSNAELSKKASGMRSPFAWASQYYTDTASGVFYSSVLQPHHYENHVYSDVTTEVQQCRRRRQRDRVTSLGAGVTPASRAGAEDEDVGHETHTATPMATGAGAATAASTRRSMERPAAESSLTAGLQPAAARKASASAGTAILTSGVGSVAPLSHSKPTQPSNERRVDDLTVKHQDDGSASYTISLTYHDSETGTSRTVNTSALPPLRRCDAGASIPGLSSLSGGTTVRAAARTKRPTTTATLALSASGAASPAHSRSVSGGEAAHAFAVSSPPARPATATMMGKSLLHSQPHQASDAEDNDNASTTLGLAQAARTGGGRPRPAPRSSISVGGSLKYCSSPAAGATAAGSASGSPACDAAAASTTTASGKVNPFAAPLPPTEPQHGPRLRGVAGRNGEMAIGAFLGGGACGKVYECLNTETGQVLAAKQIVFDA--KDRKLRTRLKQLELELEVLTLAARHHVRWIVGFFGAEKRGHSVLMYLEYCQHGSLLDYMMEGNSTDAAVWNTSSALDDATPKPSTAPADRSAGCHFDANGSPPVAAVAAWRRHTESVNEDASCLSARSPLRGLREVRESHTEAAAAPKTNSPKQQRQQQQVADGGSAAAGHAQRAEAEEAAATGHASPQVRTHREESAEGEEYPERREGDCLSTAAASVSSAASSVALSSSALDMMPLSEALHPQMPSLSIEQAQCFTKQIVEGLCFLHQHNYAHLDVKTANVLVAADDECRLADLGCAMRLQPPPPASPPPQQQGLAGMDKAVRGGANGDLDDAFSPPPYPVLVDRDAITELRGTALYMAPEMIRFESHAIGSPADVWSLGCVVMEMTTGCAPWRHIAKDKLRVLYRIGSARDELP--LPPLIRAWAEEAREW--LVREGLSATVDAEKQQDDVAACAMEAGGVAQAPRSRSASKKDREVSGVDRDSGVVGGDLDNAVSDTLDEPCSQRRRVDHGTYGCEATATPRSSSALTAKMPASVRTPATSTRGGTTSSSFARAASAMTAGLQLGESNPPDEERFFREQRHVMRLYVALQDFVTACVRVRPEDRSSTAELLRHPFLTLCKDDI 4BTF Chain:A ((7-460)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALGRFDEVLKEANQQIEKFILFHEVNEKLRDVWEELLLLLQVPASWQQEDRQDAEEDGN-ENMKVILMQLQISVEEINKTLKQCSIPQD-LQIKEIPKEHLGPPWTKLKTSKMSTIYRGEYHRSPVTIKVFNNPQAESVGIVRFTFNDEIKTMKKFD---SPNILRIFGICIDQ-----TVKPPEFSIVMEYCELGTLRELLDRE-KDLTMSVRSLLVLRAARGLYRLHHSETLHRNISSSSFLVAGGYQVKLAG---------------------------------------------FELSKTQNSISRTASSTIYVSPERLKNPFCLYDIKAEIYSFGIVLWEIATGKIPFEGCDSKKIRELVAEDKKQEPVGQDCPELLREIINECRAHEPSQRPSVDGILERLSA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1855 57149 30.81 140.42 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 30.81 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_4BTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BTF-query.scw PDB file : Tito_Scwrl_4BTF.pdb: