Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLHKTRINADPQRSPPPAPANHIRSPVGSASSQSLAISPLSTKPVDIPSPERKPNSGVVTGVSISDGGAIWDRRAQCRAPTPPPSSITVERREEAGYGAMVAKGGKASSSLSKATAGRVGSQEDDVEGGDETSSEDEEDEEEDSDEEDDEGEEGGYYDGATSDQEEDDDVDTGDSTSDDEEDEDDASTSASSSSSGSSTAPVEIGSRRTRRKMQSGKKSTQRSLKAAKNTAVPSSASSGAQWCGGPGPAAQPIRTKVSREGDVNVKVIFAARSRHCFRRYVKEVMTRFGFRKATDFDMYCIDQYGDRVDIDTEEDFEQLLDAFTEAMGVVGKDSLVGSPDTHASPPPPSLEPLHHLYGANQSTGSCGMPAVGDGSSMSRHARERAMSFSASQNHTSSFYAAKSFSLSPRLDATIASSISAGSLVEDDGKSSGSVLRLYVRYSNAYYTEHRREFEQQMNHSLLYPQSMLSNGGSGAHPPPPPPQQQQLSLGSPSSKWRLQTDQLPSGHVSPGAVGSPLARNLLAFTTCSVGSGGGDGGAISSHDELNAVHGKS-SDWAAASADSMSMQFNETL--NSNLAKTLRLDEAEP-----LDWRRMSVLGKGSFGTVYEGITQD-GKMLAVKVQELSLDDGEDAEAVKAVKAEINLMRSLKHKNIV-TYYGCQTRVLPTGNQQMEVFLELCHGGSLASLRRKFVKAKEPFSISLVRSYTRQVLEGLAYLHAQNVVHRDIKSDNVLISAMGEAKLADFGCSKRLGPATLQGMPGTLPPGAAPAAAAPSPQEAAVARAAMHQTVVGSPFFMAPEVLREDGSYTGAADIWSVGCLVLELL-GREPWDITGKNIFQIMFRISKEKGMPTGVPKKCPAILLDFFERCFQRDTKRRA-----TAVELLEHEWL-TCPDKALEE---VPPSPLSQKESPAE----------------------------------------------- 3C4Z Chain:A ((31-533)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RDRKYLARLKLPPLSKCEALRESLDLGFEGMCLEQ-PIGKRLFQQFLRTHEQHGPALQLWKDIEDYDTADDA------------LRPQKAQALRAAYLEPQAQLFCSFLDAETVARARAGGLFQPLLRAVLAHLGQAPFQEFLDSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGRGGFGEVFACQMKATGKLYACKK--LNKKRLKKRKGYQGAMVEKKILAKVHSRFIVSLAYAFETK------TDLCLVMTIMNGGDIRYHIYNVDEDNPGFQEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENVLLDDDGNVRISDLGLAVELKA-----------------------------GQTKTKGYAGTPGFMAPELLLGE-EYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKQRVLEQ-AVTYPDKFSPASKDFCEALLQKDPEKRLGFRDGSCDGLRTHPLFRDISWRQLEAGMLTPPFVPDSRTVYAKNGAFSGVAFEKADTEFFQEFASGTCPIPWQEEMIETGVFGDLNVWRP

General information: TITO was launched using: RESULT: Template: 3C4Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1973 936 0.47 2.20 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 0.47 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_3C4Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C4Z-query.scw PDB file : Tito_Scwrl_3C4Z.pdb: