Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCAAQMPPLAHIFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHASQYSFAGSS-IDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFATIHT--DSSLLLADITDKFGQRAFVGKVCMDLNDTFPE--YKE---------T-------TEESIKETERFVSEMLQKNYSRVKPIVTPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTNKTVMAHGCYLSAEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGY-SYSMLDAIRRAVMVSNILLINKVNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV 4V1X Chain:E ((21-457)) -------MQTLSIQHGTLVTMDQ-YRRVLGDSWVHVQ-DGRIVALGVHAESVP-------PPADRVIDARGKVVLPGFINAHTHVNQILLRGGPSHGRQFYDWLFNVVYPGQKAMR-PEDVAVAVRLYCAEAVRSGITTINENADSAIYPGNIEAAMAVYGEVGVRVVYARMFFDRMD-GRIQGYVDALKARSPQVELCSIMEETAVAKDRITALSDQYHGTAGGRISVWPAPATTTAVTVEGMRWAQAFARDRAVMWTLHMAESDHDERI----H-G-MSPAEYMECYGLLDERLQVAHCVYFDRKDVRLLHRHNVKVASQVVSNAYLGSGVAPVPEMVERGMAVGIGTDNGNSNDSVNMIGDMKFMAHIHRAVHR---DADVLTPEKILEMATIDGARSLGMDHEIGSIETGKRADLILLDLRHPQ--T---------TPHHHLAATIVFQAYGNEVDTVLIDGNVVMENRRL-

General information: TITO was launched using: RESULT: Template: 4V1X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2491 -66770 -26.80 -160.89 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain E : 0.68 3D Compatibility (PKB) : -26.80 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_4V1X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V1X-query.scw PDB file : Tito_Scwrl_4V1X.pdb: